Flexible Filamentous Virus Structures from Fiber Diffraction
نویسندگان
چکیده
Fiber diffraction data have been obtained from Narcissus mosaic virus, a potexvirus from the family Flexiviridae, and soybean mosaic virus, a potyvirus from the family Potyviridae. Analysis of the data in conjunction with cryo-electron microscopy data allowed us to determine the symmetry of the viruses and to make reconstructions of SMV at 19 Å resolution and of another potexvirus, papaya mosaic virus, at 18 Å resolution. These data include the first well-ordered data ever obtained for the potyviruses and the best-ordered data from the potexviruses, and offer the promise of eventual high resolution structure determinations.
منابع مشابه
Structure of flexible filamentous plant viruses.
Flexible filamentous viruses make up a large fraction of the known plant viruses, but in comparison with those of other viruses, very little is known about their structures. We have used fiber diffraction, cryo-electron microscopy, and scanning transmission electron microscopy to determine the symmetry of a potyvirus, soybean mosaic virus; to confirm the symmetry of a potexvirus, potato virus X...
متن کاملThe structure of a filamentous bacteriophage.
Many thin helical polymers, including bacterial pili and filamentous bacteriophage, have been seen as refractory to high-resolution studies by electron microscopy. Studies of the quaternary structure of such filaments have depended upon techniques such as modeling or X-ray fiber diffraction, given that direct visualization of the subunit organization has not been possible. We report the first i...
متن کاملCost Effective and Scalable Synthesis of MnO2 Doped Graphene in a Carbon Fiber/PVA: Superior Nanocomposite for High Performance Flexible Supercapacitors
In the current study, we report new flexible, free standing and high performance electrodes for electrochemical supercapacitors developed througha scalable but simple and efficient approach. Highly porous structures based on carbon fiber and poly (vinyl alcohol) (PVA) were used as a pattern. The electrochemical performances of Carbon fiber/GO-MnO2/CNT supercapacitors were characteriz...
متن کاملStructure of the coat protein in Pf1 bacteriophage determined by solid-state NMR spectroscopy.
The atomic resolution structure of Pf1 coat protein determined by solid-state NMR spectroscopy of magnetically aligned filamentous bacteriophage particles in solution is compared to the structures previously determined by X-ray fiber and neutron diffraction, the structure of its membrane-bound form, and the structure of fd coat protein. These structural comparisons provide insights into several...
متن کاملStructure of the coat protein in fd filamentous bacteriophage particles determined by solid-state NMR spectroscopy.
The atomic resolution structure of fd coat protein determined by solid-state NMR spectroscopy of magnetically aligned filamentous bacteriophage particles differs from that previously determined by x-ray fiber diffraction. Most notably, the 50-residue protein is not a single curved helix, but rather is a nearly ideal straight helix between residues 7 and 38, where there is a distinct kink, and t...
متن کامل